ABSTRACT
We analyze the recent research progress of single-cell transcriptome sequencing of nasopharyngeal carcinoma (NPC), dissect its tumor immune microenvironment, and focus on the role of myeloid cells, T cells, B cells, NK cells, and fibroblasts in the mechanism of action in NPC tissues to provide reference for the diagnosis, treatment, and prognosis prediction of NPC.
ABSTRACT
OBJECTIVE:To study the improvement effects of Rouganbao granules on liver fibrosis of model rats,and to provide experimental evidence for further development of this preparation. METHODS:Totally 45 male SD rats were divided into normal group,model group,positive control group(Fuzheng huayu capsules,0.4 g/kg)and Rouganbao granules high-dose and low-dose groups(16.8,8.4 g/kg by crude drug)according to random number table,with 9 rats in each group. Except for normal group,other groups were given CCl4sub cutaneously and high lipid diet+15% ethanol solution for 12 weeks to establish liver fibrosis model. After modeling,medication groups were given relevant medicine intragastrically,once a day,for consecutive 12 weeks. Normal group and model group were given corresponding volume of water intragastrically. The pathology change of liver tissues of rats was observed by inverted microscope. The levels of liver function indexes (ALT,AST,ALB) and liver fibrosis indexes(HA,Ⅳ-C,LN,Ⅲ-PC)were determined by ELISA. RESULTS:Compared with model group,liver cell vacuolation and liver fibrosis of rats were improved significantly in Rouganbao granule high-dose and low-dose groups;central vein was clearly visible,and no dilation and atrophy were found,especially in high-dose group;the serum levels of ALT and AST were decreased significantly,while the level of ALB was increased significantly(P＜0.05 or P＜0.01),of which the protective effect for liver was similar to positive control drug but better in increasing ALB(P＜0.05 or P＜0.01);the serum levels of HA,Ⅳ-C,LN and Ⅲ-PC were all decreased in varying degree,especially in high-dose group(P＜0.05 or P＜0.01),of which the effect of relieving liver fibrosis was similar to positive control drug. CONCLUSIONS:Rouganbao granules can improve liver function and delay the progression of liver fibrosis in model rats.
ABSTRACT
Efflux protein is an active transport protein family located on cell membranes of various human tissues,including ocular tissues.Efflux proteins can transport exogenous molecules out of the cells to function as a protective mechanism.Scientists have revealed these proteins in various human eye tissues,including cornea,iris and ciliary body,retina,as well as retinal pigment epithelium.Many studies have demonstrated that these efflux proteins have their own unique substrates and inhibitors.The effects of these efflux proteins and inhibitors can alter ocular pharmacokinetics as well as pharmacodynamics of a given therapeutics.Better understanding of the efflux proteins and their functions can provide scientific insight and guideline for development of optimal ocular drug delivery strategy.
ABSTRACT
Cell death is a major physiological or pathological phenomenon in life activities. The classic forms of cell death include apoptosis, necrosis, and autophagy. Recently, a novel type of cell death has been observed and termed as methuosis, in which excessive stimuli can induce cytoplasmic uptake and accumulation of small bubbles that gradually merge into giant vacuoles, eventually leading to decreased cellular metabolic activity, cell membrane rupture and cell death. In this article, we describe the nomenclature, morphological characteristics and underlying mechanisms of methuosis, compare methuosis with autophagy, oncosis and paraptosis, and review the related researches.
Subject(s)
Humans , Apoptosis , Autophagy , Cell Death , Cytoplasm , Necrosis , VacuolesABSTRACT
ObjectiveTo explore the clinical effect of the different surgical treatment for patients with inguinal hernia.Methods98 patients with inguinal hernia were randomly divided into two groups and 49 cases in each group.The observation group was given filling tension-free hernia repair,the control group using traditional hernia repair.The postoperative follow-up were compared in clinical efficacy and recurrence.ResultsIn the observation group and control group,the duration of postoperative pain,surgical time and patient recovery time was 93.9％ (46/49) vs 81.6％ (40/49) (x2 =2.645,P＜0.01),(45.2 ±7.2)min vs (51.4 ±6.4)min(P ＜0.05),(60.8±5.4) min vs (71.4 ± 6.7) min ( P ＜ 0.05 ),( 2.0 ± 2.1 ) d vs (4.9 ± 1.8) d ( P ＜ 0.05 ).Postoperative follow-up found that both groups of patients relapse rate was statistically significant( x2 =3.279,P ＜0.01 ).ConclusionTwo different surgical treatment of inguinal hernia,the tension-free hernia repair compared with the traditional hernia repair was more suitable for the body,surgery time and recovery time was short,reducing the relapse rate in patients with inguinal hernia,was an effective means of treatment.